Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)
![Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)](https://www.abcam.com/ps/products/251/ab251371/Images/ab251371-403367-anti-set5-antibody-epr20102-bsa-and-azide-free-western-blot.jpg "Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20102] to SET5 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Saccharomyces cerevisiae
Overview
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Product name
Anti-SET5 antibody [EPR20102] - BSA and Azide free
See all SET5 primary antibodies -
Description
Rabbit monoclonal [EPR20102] to SET5 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251371 is the carrier-free version of ab202437. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251371 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR20102 -
Isotype
IgG
Images
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Western blot - Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 secondsThis data was developed using ab202437, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)](https://www.abcam.com/ps/products/251/ab251371/Images/ab251371-399392-anti-set5-antibody-epr20102-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)All lanes : Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution
Lane 1 : Saccharomyces cerevisiae (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisiae (SET5 KO) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 secondsThis data was developed using ab202437, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Set5 KO strain (Y02901) was purchased from Euroscarf.
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![Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)](https://www.abcam.com/ps/products/251/ab251371/Images/ab251371-3-benefits-of-recombinant-antibodies.png)
Anti-SET5 antibody [EPR20102] - BSA and Azide free (ab251371)