Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (ab255272)
![Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (ab255272)](https://www.abcam.com/ps/products/255/ab255272/Images/ab255272-2-anti-serpinb3-scca-serpinb4-scca-2-antibody-epr22455-57-immunohistochemistry-pancreatic-carcinoma-human.jpg "Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (ab255272)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 whole cell lysate. Human fetal lung tissue lysate. IHC-P: Human pancreatic carcinoma and ovarian cancer tissue.
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General notes
Ab255272 is the carrier-free version of ab254255. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255272 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22455-57 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] - BSA and Azide free (ab255272)
Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma and the paired adjacent pancreas labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID: 24759783) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254255 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254255).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] - BSA and Azide free (ab255272)](https://www.abcam.com/ps/products/255/ab255272/Images/ab255272-1-anti-serpinb3-scca-serpinb4-scca-2-antibody-epr22455-57-immunohistochemistry-ovarian-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [EPR22455-57] - BSA and Azide free (ab255272)Immunohistochemical analysis of paraffin-embedded human ovarian cancer labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ovarian cancer (PMID: 23185467) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254255 for 30 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254255).
-
![Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (ab255272)](https://www.abcam.com/ps/products/255/ab255272/Images/ab255272-3-benefits-of-recombinant-antibodies.png)
Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] - BSA and Azide free (ab255272)