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Cell Biology Proteolysis / Ubiquitin Protease inhibitors Serine protease inhibitors SERPINs

Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)

Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI1A5] to SerpinB3/SCCA
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SerpinB3/SCCA antibody [OTI1A5]
    See all SerpinB3/SCCA primary antibodies
  • Description

    Mouse monoclonal [OTI1A5] to SerpinB3/SCCA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human SerpinB3/SCCA aa 1-390. Produced in HEK-293T cell (NP_008850).
    Sequence:

    MNSLSEANTKFMFDLFQQFRKSKENNIFYSPISITSALGMVLLGAKDNTA QQIKKVLHFDQVTENTTGKAATYHVDRSGNVHHQFQKLLTEFNKSTDAYE LKIANKLFGEKTYLFLQEYLDAIKKFYQTSVESVDFANAPEESRKKINSW VESQTNEKIKNLIPEGNIGSNTTLVLVNAIYFKGQWEKKFNKEDTKEEKF WPNKNTYKSIQMMRQYTSFHFASLEDVQAKVLEIPYKGKDLSMIVLLPNE IDGLQKLEEKLTAEKLMEWTSLQNMRETRVDLHLPRFKVEESYDLKDTLR TMGMVDIFNGDADLSGMTGSRGLVLSGVLHKAFVEVTEEGAEAAAATAVV GFGSSPTSTNEEFHCNHPFLFFIRQNKTNSILFYGRFSSP


    Database link: P29508
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human kidney, lung carcinoma, pancreas and bladder carcinoma tissues. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SerpinB3/SCCA cDNA.
  • General notes

    Clone OTI1A5 (formerly 1A5).

    Protein previously labeled as SerpinB3.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI1A5
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Protease inhibitors
    • Serine protease inhibitors
    • SERPINs

Images

  • Western blot - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    Western blot - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    All lanes : Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SerpinB3/SCCA cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)

    Paraffin-embedded human kidney tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)

    Paraffin-embedded human lung carcinoma tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)

    Paraffin-embedded human pancreas tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SerpinB3/SCCA antibody [OTI1A5] (ab180396)

    Paraffin-embedded human bladder carcinoma tissue stained for SerpinB3/SCCA using ab180396 at 1/150 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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