Anti-SerpinB3/SCCA antibody (ab154971)
Key features and details
- Rabbit polyclonal to SerpinB3/SCCA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SerpinB3/SCCA antibody
See all SerpinB3/SCCA primary antibodies -
Description
Rabbit polyclonal to SerpinB3/SCCA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SerpinB3/SCCA aa 89-359.
Database link: P29508 -
Positive control
- A549, HepG2 and HCT116 cell lysates; Human hepatoma tissue; A549 cells.
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General notes
Protein previously labeled as SerpinB3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SerpinB3/SCCA antibody (ab154971) at 1/1000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.
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All lanes : Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution
Lane 1 : A549 cell lysate
Lane 2 : H1299 cell lysate
Lane 3 : HCT116 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
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Anti-SerpinB3/SCCA antibody (ab154971) at 1/3000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SerpinB3/SCCA with ab154971 at 1/500 dilution.
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Immunofluorescent analysis of methanol-fixed A549 cells labeling SerpinB3/SCCA with ab154971 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.