Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serpin A5 antibody [EPR23337-112] (ab270451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23337-112] to Serpin A5
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Serpin A5 antibody [EPR23337-112]
    See all Serpin A5 primary antibodies

  • Description

    Rabbit monoclonal [EPR23337-112] to Serpin A5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum and plasma; Rat serum and plasma; Mouse serum and plasma. IHC-P: Human kidney and testis tissue.

  • General notes

    This antibody, ab270451 (EPR23337-112),  is a different clone to ab270450 (EPR23337-69).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    Western blot - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    All lanes : Anti-Serpin A5 antibody [EPR23337-112] (ab270451) at 1/1000 dilution

    Lane 1 : Human serum lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Rat serum lysate
    Lane 4 : Rat plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 46 kDa
    Observed band size: 45-52 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature. Bands around 50 kDa represent the intact and cleaved forms (PMID: 22206708).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Serpin A5 with ab270451 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney (PMID: 14696115). The section was incubated with ab270451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    Western blot - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    All lanes : Anti-Serpin A5 antibody [EPR23337-112] (ab270451) at 1/1000 dilution

    Lane 1 : Mouse serum lysate
    Lane 2 : Mouse plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 46 kDa
    Observed band size: 150,52 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature. Bands detected between 80 kDa and 120 kDa could be either the dimeric form or serpin A5 in complex with a serine protease such as urokinase or plasma kallikrein. Bands around 50 kDa represent the intact and cleaved forms (PMID: 22206708).

    Exposure time: 3 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-112] (ab270451)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serpin A5 with ab270451 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis (PMID: 1372913). The section was incubated with ab270451 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Serpin A5 antibody [EPR23337-112] (ab270451)
    Anti-Serpin A5 antibody [EPR23337-112] (ab270451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.