Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12122] to Septin 2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Septin 2 antibody [EPR12122] - BSA and Azide free
    See all Septin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12122] to Septin 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250933 is the carrier-free version of ab187654.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR12122

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Immunocytochemistry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)

    This data was developed using ab187654, the same antibody clone in a different buffer formulation.

    Immunofluorescence analysis of MCF7 cells labeling Septin 2 using ab187654 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
  • Western blot - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Western blot - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using ab187654, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Western blot - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/1000 dilution

    Lane 1 : Raw 264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa



    This data was developed using ab187654, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    This data was developed using ab187654, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human breast carcinoma tissue labeling Septin 2 with ab187654 at a 1/1000 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Immunocytochemistry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    This data was developed using ab187654, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, MCF-7 cell line labeling Septin 2 with ab187654 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200 (left panel). In blue DAPI staining (right panel).
  • Flow Cytometry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Flow Cytometry - Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    This data was developed using ab187654, the same antibody clone in a different buffer formulation.Flow cytometry analysis of paraformaldehyde-fixed Jurkat cells labeling Septin 2 with ab187654 at a 1/120 dilution and secondary antibody FITC goat anti-rabbit IgG (red) at a 1/150 dilution, or negative control rabbit IgG (green).
  • Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)
    Anti-Septin 2 antibody [EPR12122] - BSA and Azide free (ab250933)

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