Anti-Septin 11 antibody (ab183529)
Key features and details
- Rabbit polyclonal to Septin 11
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Septin 11 antibody
See all Septin 11 primary antibodies -
Description
Rabbit polyclonal to Septin 11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Septin 11 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_060713.1.
Database link: Q9NVA2 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK1, and mouse NIH3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab183529 was affinity purified using an epitope specific to Septin 11 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Septin 11 antibody (ab183529)
Detection of Septin 11 by Western Blot of Immunoprecipitate.
ab183529 at 0.2µg/ml labeling Septin 11 in HeLa whole cell lysate immunoprecipitated using ab183529 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds. -
Western blot - Anti-Septin 11 antibody (ab183529)All lanes : Anti-Septin 11 antibody (ab183529) at 0.2 µg
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 3 minutes
