Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Seladin 1 antibody [EPR10743] (ab181062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10743] to Seladin 1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Seladin 1 antibody [EPR10743]
    See all Seladin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10743] to Seladin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, A375 and HepG2 cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)
    Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)
    All lanes : Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/2000 dilution

    Lane 1 : Wild-type A431 whole cell lysate
    Lane 2 : DHCR24 knockout A431 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 51 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab181062 observed at 51 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab181062 was shown to react with DHCR24 in A431 wild-type cells in Western blot. Loss of signal was observed when DHCR24 knockout sample was used. A431 wild-type and DHCR24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181062 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)
    Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)
    All lanes : Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 60 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Anti-Seladin 1 antibody [EPR10743] (ab181062)
    Anti-Seladin 1 antibody [EPR10743] (ab181062)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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