Anti-Seladin 1 antibody [EPR10743] (ab181062)
![Anti-Seladin 1 antibody [EPR10743] (ab181062)](https://www.abcam.com/ps/products/181/ab181062/Images/ab181062-380875-anti-seladin-1-antibody-epr10743-western-blot-a431-wt-a431-dhcr24-knockout-hepg2-shsy5y.jpg "Anti-Seladin 1 antibody [EPR10743] (ab181062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10743] to Seladin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Seladin 1 antibody [EPR10743]
See all Seladin 1 primary antibodies -
Description
Rabbit monoclonal [EPR10743] to Seladin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, A375 and HepG2 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10743 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)All lanes : Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/2000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : DHCR24 knockout A431 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab181062 observed at 51 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181062 was shown to react with DHCR24 in A431 wild-type cells in Western blot. Loss of signal was observed when DHCR24 knockout sample was used. A431 wild-type and DHCR24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab181062 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)](https://www.abcam.com/ps/products/181/ab181062/Images/ab181062-213192-anti-seladin-1-antibody-epr10743-western-blot.jpg)
Western blot - Anti-Seladin 1 antibody [EPR10743] (ab181062)All lanes : Anti-Seladin 1 antibody [EPR10743] (ab181062) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 60 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Anti-Seladin 1 antibody [EPR10743] (ab181062)](https://www.abcam.com/ps/products/181/ab181062/Images/ab181062-3-benefits-of-recombinant-antibodies.png)
Anti-Seladin 1 antibody [EPR10743] (ab181062)
