Anti-Secretory phospholipase A2 antibody (ab23705)
Key features and details
- Rabbit polyclonal to Secretory phospholipase A2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Secretory phospholipase A2 antibody
See all Secretory phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Secretory phospholipase A2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Human recombinant sPLA2 (Type IIa).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Secretory phospholipase A2 antibody (ab23705)
Western blot detection of human secretory phospholipase A2 (25
ab23705 at 1/1000 western blot detection of 0.25 ug of human secretory phospolipase A2.µ g) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretory phospholipase A2 antibody (ab23705)IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.