Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22326-112] to Scn1a - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), IHC-Fr, ICC
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free
    See all Scn1a primary antibodies

  • Description

    Rabbit monoclonal [EPR22326-112] to Scn1a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), IHC-Fr, ICCmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebellum, Mouse hippocampus tissues. IHC-Fr: Mouse hippocampus (fresh) tissue. ICC: Mouse primary neuron cells. Flow Cyt: Mouse primary neuron cells.

  • General notes

    ab281839 is the carrier-free version of ab222091. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281839 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22326-112

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Scn1a with ab222091 at 1/1000 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labelling Scn1a with ab222091 at 1/1000 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse hippocampus. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling Scn1a with ab222091 at 1/500 dilution (0.54 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cardiac muscle. The section was incubated with ab222091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Immunohistochemistry (Frozen sections) - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh) tissue labeling Scn1a with ab222091 at 1/100 dilution (5.4 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

  • Immunocytochemistry - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Immunocytochemistry - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Scn1a with ab222091 at 1/50 dilution (10.8 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Flow Cytometry - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Flow Cytometry - Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

    This data was developed using ab222091, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Scn1a with ab222091 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)
    Anti-Scn1a antibody [EPR22326-112] - BSA and Azide free (ab281839)

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