Anti-Scn1a antibody (ab24820)
Key features and details
- Rabbit polyclonal to Scn1a
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Scn1a antibody
See all Scn1a primary antibodies -
Description
Rabbit polyclonal to Scn1a -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Zebrafish
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Immunogen
Synthetic peptide within Human Scn1a aa 1450-1550. The exact sequence is proprietary.
Database link: P35498 -
General notes
Ab with sodium azide is stable for 24 months when stored at 2-8 °C.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scn1a antibody (ab24820)Staining of human cerebellum with anti-sodium channel antibody. The tissue was boiled in 10mM citrate buffer, pH 6.0 for 10 mins followed by cooling at room temparture for 20 mins. The working dilution for the antibody is 1:200 for 30 min at room temparture.
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Immunocytochemistry/ Immunofluorescence - Anti-Scn1a antibody (ab24820)ICC/IF image of ab24820 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24820, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
