Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal
    See all Scavenging Receptor SRB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7 and HeLa whole cell lysates; Human fetal kidney lysate; Human and Mouse liver tissues; MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    All lanes : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on human liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Scavenging Receptor SRB2 with ab196651 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

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