Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13034] to SAMHD1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free
    See all SAMHD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13034] to SAMHD1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251073 is the carrier-free version of ab191420. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251073 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)

    This data was developed using ab191420, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: SAMHD1 knockout HAP1 cell lysate (20 µg) 

    Lane 3: HepG2 cell lysate (20 µg)

    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab191420 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191420 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab191420 and ab8245 (loading control to GAPDH) dilted to 1/500 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/10000 dilution + THP1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa



    This data was developed using ab191420, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    Western blot - Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    All lanes : Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa



    This data was developed using ab191420, the same antibody clone in a different buffer formulation.

     

  • Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)
    Anti-SAMHD1 antibody [EPR13034] - BSA and Azide free (ab251073)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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