Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SAMD9 antibody [EPR13603] (ab180575)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13603] to SAMD9
Suitable for: Flow Cyt, WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-SAMD9 antibody [EPR13603]
See all SAMD9 primary antibodies
Description

Rabbit monoclonal [EPR13603] to SAMD9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A549, A431 and MCF7 cell lysates. Flow Cyt: A431 and MCF7 cells. ICC/IF: A431 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13603
Isotype

IgG
Research areas

Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : SAMD9 knockout A549 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human skeletal muscle cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 184 kDa
Observed band size: 184 kDa

Lanes 1-4: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180575 Anti-SAMD9 antibody [EPR13603] was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267038 (knockout cell lysate ab257656) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575)

Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-SAMD9 antibody [EPR13603] (ab180575)

Flow Cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : SAMD9 knockout A549 cell lysate
Lane 3 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 184 kDa
Observed band size: 184 kDa

Lanes 1-3: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control ab8245 observed at 36 kDa.

ab180575 Anti-SAMD9 antibody [EPR13603] was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267039 (knockout cell lysate ab257657) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 184 kDa
Observed band size: 184 kDa
Western blot - Anti-SAMD9 antibody [EPR13603] (ab180575)

All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/10000 dilution (unpurified)

Lane 1 : A431 cell lysate with NFDM/TBST
Lane 2 : MCF-7 cell lysate with NFDM/TBST

Lysates/proteins at 20 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

Predicted band size: 184 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] (ab180575)

Immunofluorescence analysis of 4% paraformaldehyde-A431 cells labeling SAMD9 with ab180575 (unpurified) (green) at 1/100 dilution. Dapi counterstain (blue). Goat anti rabbit IgG (Dylight 488) at a dilution of 1/200 was used as a secondary antibody.
Flow Cytometry - Anti-SAMD9 antibody [EPR13603] (ab180575)

Flow cytometric analysis of 2% paraformaldehyde-fixed A431 cells labeling SAMD9 with ab180575 (unpurified) at 1/50 dilution, or Rabbit monoclonal IgG Isotype control.
Anti-SAMD9 antibody [EPR13603] (ab180575)

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