Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5250] to S100 alpha - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-S100 alpha antibody [EPR5250] - BSA and Azide free
    See all S100 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR5250] to S100 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248534 is the carrier-free version of ab133519. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248534 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution (purified)

    Lane 1 : Rat heart lysates
    Lane 2 : Human fetal heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 10 kDa



    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling S100 alpha with purified ab133519 at 1:100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Immunohistochemistry analysis of paraffin embedded Human endometrial carcinoma tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution + Mouse heart lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    All lanes : Anti-S100 alpha antibody [EPR5250] (ab133519) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : C2C12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 10 kDa



    This data was developed using ab133519, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    This data was developed using ab133519, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of paraffin embedded Human heart muscle tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    This data was developed using ab133519, the same antibody clone in a different buffer formulation.Immunohistochemistry analysis of paraffin embedded Mouse heart tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)
    Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (ab248534)

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