Anti-RYBP antibody (ab5976)
Key features and details
- Rabbit polyclonal to RYBP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RYBP antibody
See all RYBP primary antibodies -
Description
Rabbit polyclonal to RYBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
TPKGDMSAVNDESF
, corresponding to amino acids 215-228 of Human DEDAF. -
Positive control
- A549, HepG2 or 3T3 whole cell lysate.
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General notes
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
DEDAF Antibody is affinity chromatography purified via peptide column. -
Primary antibody notes
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RYBP antibody (ab5976)
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.µ g /ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RYBP antibody (ab5976)Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976) This image is courtesy of an Abreview submitted by Dr. Ligia Tavaresab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.
