Anti-RRAGC antibody - BSA and Azide free (ab206864)
Key features and details
- Rabbit polyclonal to RRAGC - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-RRAGC antibody - BSA and Azide free
See all RRAGC primary antibodies -
Description
Rabbit polyclonal to RRAGC - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length native protein (purified) (GST-tag) corresponding to Human RRAGC aa 1 to the C-terminus.
Database link: Q9HB90 -
Positive control
- HeLa, MCF7, MOLT4, NIH 3T3 and CHO cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)
ab206864 is filter-sterilized -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab206864 is a purified IgG fraction. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RRAGC antibody - BSA and Azide free (ab206864)All lanes : Anti-RRAGC antibody - BSA and Azide free (ab206864) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : CHO cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : anti-rabbit IgG conjugated with HRP at 1/10000 dilution
Predicted band size: 44 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RRAGC antibody - BSA and Azide free (ab206864)Immunofluorecent analysis of HeLa cells (fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100) labeling RRAGC with ab206864 at a 1/100 dilution. Secondary staining with anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (red), was used at 1/1000 dilution. DNA was stained with DAPI (blue).
