Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS8 antibody [EPR17334] (ab201454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17334] to RPS8
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS8 antibody [EPR17334]
    See all RPS8 primary antibodies

  • Description

    Rabbit monoclonal [EPR17334] to RPS8

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HEK293, A549, C6, RAW 264.7 and PC-12 whole cell lysates; Human cerebellum and pancreas lysates. ICC/IF: A549 and Jurkat cells. Flow Cyt: A549 cells. IP: HEK293 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17334

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    All lanes : Anti-RPS8 antibody [EPR17334] (ab201454) at 1/10000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    All lanes : Anti-RPS8 antibody [EPR17334] (ab201454) at 1/10000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human pancreas lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    Western blot - Anti-RPS8 antibody [EPR17334] (ab201454)
    All lanes : Anti-RPS8 antibody [EPR17334] (ab201454) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (ab201454)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (ab201454)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/600 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on A549 cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
    -ve control 1: ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
    -ve control 2: ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (ab201454)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS8 antibody [EPR17334] (ab201454)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton-X100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling RPS8 with ab201454 at 1/600 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is stained with ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution (red).
    -ve control 1: ab201454 at 1/600 dilution followed by AlexaFluor®594 Goat anti-Mouse secondary antibody (ab150120) at 1/500 dilution.
    -ve control 2: ab7291 anti-Tubulin (mouse mAb) at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/500 dilution.

  • Flow Cytometry - Anti-RPS8 antibody [EPR17334] (ab201454)
    Flow Cytometry - Anti-RPS8 antibody [EPR17334] (ab201454)

    Flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling RPS8 with ab201454 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-RPS8 antibody [EPR17334] (ab201454)
    Immunoprecipitation - Anti-RPS8 antibody [EPR17334] (ab201454)

    RPS8 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab201454 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab201454 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab201454 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201454 in HEK293 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-RPS8 antibody [EPR17334] (ab201454)
    Anti-RPS8 antibody [EPR17334] (ab201454)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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