Anti-RPS20 antibody [EPR8716] (ab133776)
![Anti-RPS20 antibody [EPR8716] (ab133776)](https://www.abcam.com/ps/products/133/ab133776/Images/ab133776-147910-anti-rps20-antibody-epr8716-western-blot.jpg "Anti-RPS20 antibody [EPR8716] (ab133776)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8716] to RPS20
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-RPS20 antibody [EPR8716]
See all RPS20 primary antibodies -
Description
Rabbit monoclonal [EPR8716] to RPS20 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
A synthetic peptide corresponding to residues in Human RPS20 (UniProt ID: P60866)
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Positive control
- HeLa, A431, HUVEC and Human placenta lysates. Human cervical carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.51 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8716 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPS20 antibody [EPR8716] (ab133776)All lanes : Anti-RPS20 antibody [EPR8716] (ab133776) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : Human placenta cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 13 kDa
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![Flow Cytometry - Anti-RPS20 antibody [EPR8716] (ab133776)](https://www.abcam.com/ps/products/133/ab133776/Images/ab133776-278326-anti-rps20-antibody-epr8716-flow-cytometry.jpg)
Flow Cytometry - Anti-RPS20 antibody [EPR8716] (ab133776)Flow Cytometry analysis of A431 cells (human epidermoid carcinoma) labeling RPS20 with purified ab9133776 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] (ab133776)](https://www.abcam.com/ps/products/133/ab133776/Images/ab133776-147909-anti-rps20-antibody-epr8716-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS20 antibody [EPR8716] (ab133776)Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling RPS20 using ab133776 at 1/100 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-RPS20 antibody [EPR8716] (ab133776)](https://www.abcam.com/ps/products/133/ab133776/Images/ab133776-147908-anti-rps20-antibody-epr8716.jpg)
OI-RD Scanning - Anti-RPS20 antibody [EPR8716] (ab133776) -
![Anti-RPS20 antibody [EPR8716] (ab133776)](https://www.abcam.com/ps/products/133/ab133776/Images/ab133776-5-benefits-of-recombinant-antibodies.png)
Anti-RPS20 antibody [EPR8716] (ab133776)
