Anti-RPF2 antibody [EP10252] (ab180604)
![Anti-RPF2 antibody [EP10252] (ab180604)](https://www.abcam.com/ps/products/180/ab180604/Images/ab180604-211980-icc.jpg "Anti-RPF2 antibody [EP10252] (ab180604)")
Key features and details
- Rabbit monoclonal [EP10252] to RPF2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPF2 antibody [EP10252]
See all RPF2 primary antibodies -
Description
Rabbit monoclonal [EP10252] to RPF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, Jurkat and A549 lysates; Human kidney cell carcinoma tissue; 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP10252 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RPF2 antibody [EP10252] (ab180604)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling RPF2 with ab180604 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (left panel). Dapi stain (right panel).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPF2 antibody [EP10252] (ab180604)](https://www.abcam.com/ps/products/180/ab180604/Images/ab180604-211979-ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPF2 antibody [EP10252] (ab180604)Immunohistochemical analysis of paraffin-embedded Human kidney cell carcinoma labeling RPF2 with ab180604 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-RPF2 antibody [EP10252] (ab180604)](https://www.abcam.com/ps/products/180/ab180604/Images/ab180604-211978-wb.jpg)
Western blot - Anti-RPF2 antibody [EP10252] (ab180604)All lanes : Anti-RPF2 antibody [EP10252] (ab180604) at 1/20000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
