Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI5G7] to ROD1
Suitable for: IHC-P, WB
Reacts with: Human
Isotype: IgG2a

Product name

Anti-ROD1 antibody [OTI5G7]
See all ROD1 primary antibodies
Description

Mouse monoclonal [OTI5G7] to ROD1
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human ROD1 aa 300 to the C-terminus. Produced in E.coli (NP_005147).
Database link: O95758-3

Run BLAST with Run BLAST with
Positive control
- WB: pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate; Jurkat, A549 and HeLa cell lysates. IHC-P: Human breast, breast adenocarcinoma, colon, colon adenocarcinoma, kidney, liver, ovary adenocarinoma, thyroid, endometrium adenocarcinoma, prostate carcinoma and spleen tissues.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

OTI5G7
Isotype

IgG2a
Research areas

Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407)

All lanes : Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate

Lysates/proteins at 5 µg per lane.

Predicted band size: 60 kDa
Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407)

All lanes : Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/500 dilution

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lysates/proteins at 35 µg per lane.

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human breast tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human breast adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human colon tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human colon adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human kidney tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human liver tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human ovary adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human thyroid tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human prostate carcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)

Paraffin-embedded human spleen tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

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