Anti-ROD1 antibody [OTI5G7] (ab236407)
Key features and details
- Mouse monoclonal [OTI5G7] to ROD1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ROD1 antibody [OTI5G7]
See all ROD1 primary antibodies -
Description
Mouse monoclonal [OTI5G7] to ROD1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human ROD1 aa 300 to the C-terminus. Produced in E.coli (NP_005147).
Database link: O95758-3 -
Positive control
- WB: pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate; Jurkat, A549 and HeLa cell lysates. IHC-P: Human breast, breast adenocarcinoma, colon, colon adenocarcinoma, kidney, liver, ovary adenocarinoma, thyroid, endometrium adenocarcinoma, prostate carcinoma and spleen tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5G7 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa
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All lanes : Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 60 kDa
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Paraffin-embedded human breast tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate carcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human spleen tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.