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Cell Biology Other Antibodies

Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)

Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12940] to RNF214 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RNF214 antibody [EPR12940] - BSA and Azide free
    See all RNF214 primary antibodies
  • Description

    Rabbit monoclonal [EPR12940] to RNF214 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RNF214. The exact sequence is proprietary.
    Database link: Q8ND24

  • General notes

    ab250252 is the carrier-free version of ab180850 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250252 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12940
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Images

  • Immunocytochemistry - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    Immunocytochemistry - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    This data was developed using ab180850, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.
  • Western blot - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    Western blot - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    All lanes : Anti-RNF214 antibody [EPR12940] (ab180850) at 1/1000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : HT-1376 lysates
    Lane 3 : HepG2 lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa



    This data was developed using ab180850, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    Immunoprecipitation - Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    This data was developed using ab180850, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.
  • Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)
    Anti-RNF214 antibody [EPR12940] - BSA and Azide free (ab250252)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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