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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6D7] to RNA polymerase II CTD repeat YSPTSPS (phospho T4) - BSA and Azide free
  • Suitable for: ELISA, WB, Dot blot
  • Reacts with: Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free
    See all RNA polymerase II CTD repeat YSPTSPS primary antibodies

  • Description

    Rat monoclonal [6D7] to RNA polymerase II CTD repeat YSPTSPS (phospho T4) - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: PC-12 whole cell lysate.

  • General notes

    ab255845 is the carrier-free version of ab252851. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255845 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate (Untreated membrane)
    Lane 2 : PC-12 whole cell lysate (Phosphatase treated membrane)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 217 kDa
    Observed band size: 260 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was produced using the same clone but in a different formulation, ab252851.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22745433 and 23071310).

    This blot was developed using a higher sensitivity ECL substrate.
    This antibody reacts with rat on WB.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)
    ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)

    This data was produced using the same clone but in a different formulation, ab252851.

    ab252851 used at 0-1000 ng/ml.

    Antigen (phospho S2, phospho S5, phospho T4, phospho Y1, phospho S7, non-phospho) used at 500 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)
    Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)

    This data was produced using the same clone but in a different formulation, ab252851.

    ab252851 used at 1/1000 dilution, follwed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution.

    Lane 1: RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide

    Lane 2: RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide

    Lane 3: RNA polymerase II CTD repeat YSPTSPS (phospho T4) peptide

    Lane 4: RNA polymerase II CTD repeat YSPTSPS (phospho Y1) peptide

    Lane 5: RNA polymerase II CTD repeat YSPTSPS (phospho S7) peptide

    Lane 6: RNA polymerase II CTD repeat YSPTSPS (non-phospho) peptide

    Blcoking/Dilution buffer: 5% NFDM/TBST.

    Exposure time 3 mins.

  • Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)
    Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] - BSA and Azide free (ab255845)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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