Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [6D7] to RNA polymerase II CTD repeat YSPTSPS (phospho T4)
Suitable for: ELISA, WB, Dot blot
Reacts with: Rat, Human

Product name

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7]
See all RNA polymerase II CTD repeat YSPTSPS primary antibodies
Description

Rat monoclonal [6D7] to RNA polymerase II CTD repeat YSPTSPS (phospho T4)
Host species

Rat
Tested Applications & Species

Application Species
WB
Rat

See all applications and species data
Immunogen

The details of the immunogen for this antibody are not available.
Positive control
- WB: PC-12 whole cell lysate.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
WB	Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59.94% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

6D7
Isotype

IgG2b
Research areas

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851)

All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851) at 1/1000 dilution

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate (Untreated membrane)
Lane 2 : PC-12 whole cell lysate (Phosphatase treated membrane)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 217 kDa
Observed band size: 260 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22745433 and 23071310).

This blot was developed using a higher sensitivity ECL substrate.
This antibody reacts with rat on WB.

Blocking/Dilution buffer: 5% NFDM/TBST.
ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851)

ab252851 used at 0-1000 ng/ml.

Antigen (phospho S2, phospho S5, phospho T4, phospho Y1, phospho S7, non-phospho) used at 500 ng/ml.

Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851)

ab252851 used at 1/1000 dilution, followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution.

Lane 1: RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide

Lane 2: RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide

Lane 3: RNA polymerase II CTD repeat YSPTSPS (phospho T4) peptide

Lane 4: RNA polymerase II CTD repeat YSPTSPS (phospho Y1) peptide

Lane 5: RNA polymerase II CTD repeat YSPTSPS (phospho S7) peptide

Lane 6: RNA polymerase II CTD repeat YSPTSPS (non-phospho) peptide

Blcoking/Dilution buffer: 5% NFDM/TBST.

Exposure time 3 mins.
Anti-RNA polymerase II CTD repeat YSPTSPS (phospho T4) antibody [6D7] (ab252851)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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