Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg / 10⁶  cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Formalin-fixed, paraffin-embedded human lymph node tissue stained for Retinoid X Receptor beta/RXRB using ab277104 at 2 µg/ml in immunohistochemical analysis.

Flow cytometry analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg / 10⁶ cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277104.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.