Anti-Renin antibody [EPR20693] (ab212197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20693] to Renin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Renin antibody [EPR20693]
See all Renin primary antibodies -
Description
Rabbit monoclonal [EPR20693] to Renin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseRatIHC-P MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human and mouse kidney tissues. IHC-Fr: Mouse and rat kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20693 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Renin with ab212197 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of human kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of mouse kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Renin antibody [EPR20693] (ab212197)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Renin with ab212197 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on juxtaglomerular cells of mouse kidney (PMID: 1898348). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval performed using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Renin with ab212197 at 1/6000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in juxtaglomerular cells of rat kidney tissue section (PMID: 27080979).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval performed using Tris-EDTA buffer (pH9.0) (ab94681).
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