Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
![Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)](https://www.abcam.com/ps/products/211/ab211925/Images/ab211925-393690-anti-alas1-antibody-epr10247-mitochondrial-marker-western-blot-human-knockout-lysate.jpg "Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10247] to Alas1 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Alas1 antibody [EPR10247] - BSA and Azide free
See all Alas1 primary antibodies -
Description
Rabbit monoclonal [EPR10247] to Alas1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2 and HEK293T cell lysates. ICC/IF: HepG2 cells.
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General notes
Ab211925 is the carrier-free version of ab154860. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab211925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10247 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)All lanes : Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALAS1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 71 kDa
Observed band size: 71 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab154860).
Lanes 1-4: Merged signal (red and green). Green - ab154860 observed at 71 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154860 Anti-Alas1 antibody [EPR10247] was shown to specifically react with Alas1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266473 (knockout cell lysate ab257348) was used. Wild-type and Alas1 knockout samples were subjected to SDS-PAGE. ab154860 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)](https://www.abcam.com/ps/products/211/ab211925/Images/ab211925-326852-anti-alas1-antibody-epr10247-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154860).
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![Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)](https://www.abcam.com/ps/products/211/ab211925/Images/ab211925-326851-anti-alas1-antibody-epr10247-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925) -
![Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)](https://www.abcam.com/ps/products/211/ab211925/Images/ab211925-4-benefits-of-recombinant-antibodies.png)
Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
