Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10247] to Alas1 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Alas1 antibody [EPR10247] - BSA and Azide free
    See all Alas1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10247] to Alas1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2 and HEK293T cell lysates. ICC/IF: HepG2 cells.

  • General notes

    Ab211925 is the carrier-free version of ab154860. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab211925 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
    Western blot - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
    All lanes : Anti-Alas1 antibody [EPR10247] - Mitochondrial Marker (ab154860) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ALAS1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab154860).

    Lanes 1-4: Merged signal (red and green). Green - ab154860 observed at 71 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab154860 Anti-Alas1 antibody [EPR10247]  was shown to specifically react with Alas1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266473 (knockout cell lysate ab257348) was used.  Wild-type and Alas1 knockout samples were subjected to SDS-PAGE.  ab154860 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
    Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Alas1 with purified ab154860 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154860).

  • Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
    Immunocytochemistry/ Immunofluorescence - Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)

    Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labeling Alas1 with unpurified ab154860 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154860).

  • Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)
    Anti-Alas1 antibody [EPR10247] - BSA and Azide free (ab211925)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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