Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPRR18188] to REG3A + REG3G
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-REG3A + REG3G antibody [EPRR18188]
    See all REG3A + REG3G primary antibodies

  • Description

    Rabbit monoclonal [EPRR18188] to REG3A + REG3G

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine lysate; REG3A recombinant protein fragment; REG3G recombinant protein fragment. IHC-P: Human pancreas tissue. ICC/IF: BxPC-3 and HT-29 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPRR18188

  • Isotype

    IgG

Images

  • Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    All lanes : Anti-REG3A + REG3G antibody [EPRR18188] (ab202057) at 1/1000 dilution

    Lane 1 : Human small intestine lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 16 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literatures PMID: 12890698 & 22943287 for REG3A and PMID: 22252863 & 21254165 for REG3G.
    Please note both Human fetal liver and brain represent negative controls for REG3A and REG3G.

  • Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Western blot - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    All lanes : Anti-REG3A + REG3G antibody [EPRR18188] (ab202057) at 1/10000 dilution

    Lane 1 : REG3A recombinant protein fragment
    Lane 2 : REG3G recombinant protein fragment

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling REG3G + REG3A with ab202057 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Immunocytochemistry/ Immunofluorescence - Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cells) cells labeling REG3G + REG3A with ab202057 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab202057 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)
    Anti-REG3A + REG3G antibody [EPRR18188] (ab202057)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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