Anti-RBM26 antibody (ab74198)
Key features and details
- Rabbit polyclonal to RBM26
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RBM26 antibody -
Description
Rabbit polyclonal to RBM26 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 450 and 500 of human RBM26 (NP_071401.3)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74198 was affinity purified using an epitope specific to RBM26 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RBM26 antibody (ab74198)All lanes : Anti-RBM26 antibody (ab74198) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
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Immunoprecipitation - Anti-RBM26 antibody (ab74198)Detection of RBM26 by Western Blot of Immunprecipitate.
ab74198, at 1µg/ml, staining RBM26 in HeLa whole cell lysate immunoprecipitated using ab74198 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.