Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RASSF1a antibody [3F3] (ab23950)

Key features and details

  • Mouse monoclonal [3F3] to RASSF1a
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RASSF1a antibody [3F3]
    See all RASSF1a primary antibodies

  • Description

    Mouse monoclonal [3F3] to RASSF1a

  • Host species

    Mouse

  • Specificity

    In many cases, the results of staining showed speckled pattern or weak staining in cytoplasm but we couldn’t observe microtubule stained with this antibody. In mitotic cell, we could observe the mitotic spindles stained with this antibody and so thought it was kinetochore stained specifically.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant human RASSF1A purified from E. coli.

  • Epitope

    ab23950 specifically recognises the C1 domain (52-101aa) of RASSF1a

  • Positive control

    • WB: HeLa lysate, 293cells transfected with HA-tagged RASSF 1A gene ICC: A431 cells

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (ab23950)
    Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (ab23950)

    Immunocytochemistry/ Immunofluorescence analysis of RASSF1a in HeLa cells. The cell was stained with ab23950 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    Anti-RASSF1a antibody [3F3] (ab23950) at 1/500 dilution + HeLa cell lysate at 40 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 39 kDa

  • Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : RASSF1A transfected 293T cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 39 kDa

  • Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

    Lane 1 : Hela (H) cell extract
    Lane 2 : Mouse brain (MB) extract
    Lane 3 : Mouse liver (ML) extract
    Lane 4 : Mouse kidney (MK) extract

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-mouse HRP conjugate

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 40,43 kDa
    why is the actual band size different from the predicted?



    Arrows indicate RASSF1A protein (about 40kDa in Hela cells, and 43kDa in other tissues from mice).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (ab23950)
    Immunocytochemistry/ Immunofluorescence - Anti-RASSF1a antibody [3F3] (ab23950)

    A431 cells were labeled with anti-RASSF1a(clone 3F3) ab23950. Detection was done using a biotinylated secondary antibody and Texas-red conjugated streptavidin.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    Western blot - Anti-RASSF1a antibody [3F3] (ab23950)
    All lanes : Anti-RASSF1a antibody [3F3] (ab23950) at 1/1000 dilution

    Lane 1 : HEK293 cells
    Lane 2 : A549 cells
    Lane 3 : HeLa cells

    Predicted band size: 39 kDa



    Proteins were visualised using a goat anti-mouse secondary antibody conjugation to HRP and a ECL detection system.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-RASSF1a antibody [3F3] (ab23950)
    Flow Cytometry - Anti-RASSF1a antibody [3F3] (ab23950)

    Overlay histogram showing HeLA cells stained with ab23950 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23950, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RASSF1a antibody [3F3] (ab23950)

© 2021 Astana Biomed Group LLP. All rights reserved.