Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ras antibody [EPR18713-13] (ab206969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18713-13] to Ras
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Ras antibody [EPR18713-13]
    See all Ras primary antibodies

  • Description

    Rabbit monoclonal [EPR18713-13] to Ras

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human full length K, H and N RAS protein, Human fetal brain and fetal kidney lysates; HeLa, A431, C6 and NIH/3T3 whole cell lysates; Mouse and rat brain lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: A549 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    All lanes : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/5000 dilution

    All lanes : Recombinant Human RAS full length protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2 and 3: 5 seconds.

    Recombinant Human RAS full length protein contains aa1-189 with an N-terminal His-Tag®. Recombinant Human HRAS full length protein contains aa1-189 with an N-terminal His-Tag®. Recombinant Human NRAS full length protein contains aa1-189 with an N-terminal His-Tag®. All three recombinant proteins were made in house.

  • Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Lane 1 : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/2000 dilution
    Lane 2 : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    All lanes : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Lane 1 : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/2000 dilution
    Lane 2 : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    Western blot - Anti-Ras antibody [EPR18713-13] (ab206969)
    All lanes : Anti-Ras antibody [EPR18713-13] (ab206969) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR18713-13] (ab206969)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR18713-13] (ab206969)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling RAS with ab206969 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm and nuclear staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab206969 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR18713-13] (ab206969)
    Immunocytochemistry/ Immunofluorescence - Anti-Ras antibody [EPR18713-13] (ab206969)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling RAS with ab206969 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm and nuclear staining on NIH/3T3 cells line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab206969 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry - Anti-Ras antibody [EPR18713-13] (ab206969)
    Flow Cytometry - Anti-Ras antibody [EPR18713-13] (ab206969)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling RAS with ab206969 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Ras antibody [EPR18713-13] (ab206969)
    Immunoprecipitation - Anti-Ras antibody [EPR18713-13] (ab206969)

    RAS was immunoprecipitated from 1mg of A549 (Human lung carcinoma) whole cell lysate with ab206969 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab206969 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A549 whole cell lysate 10ug (Input).

    Lane 2: ab206969 IP in A549 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206969 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-Ras antibody [EPR18713-13] (ab206969)
    Anti-Ras antibody [EPR18713-13] (ab206969)

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