Anti-Ramp4-2 antibody [EPR14338] (ab184571)
![Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.com/ps/products/184/ab184571/Images/ab184571-217139-ab184571d.jpg "Anti-Ramp4-2 antibody [EPR14338] (ab184571)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14338] to Ramp4-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Ramp4-2 antibody [EPR14338]
See all Ramp4-2 primary antibodies -
Description
Rabbit monoclonal [EPR14338] to Ramp4-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, LnCaP and SW480 cell lysates; Human liver tissue; SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14338 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Ramp4-2 antibody [EPR14338] (ab184571)
Immunofluorescent analysis of 4% paraformaldehyde-fixed SW480 cells labeling Ramp4-2 with ab184571 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 diution. Counter stained with Dapi (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.com/ps/products/184/ab184571/Images/ab184571-217138-ab184571c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ramp4-2 antibody [EPR14338] (ab184571)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ramp4-2 with ab184571 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.com/ps/products/184/ab184571/Images/ab184571-217137-ab184571b.jpg)
Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)All lanes : Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/100000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa
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![Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.com/ps/products/184/ab184571/Images/ab184571-217136-ab184571a.jpg)
Western blot - Anti-Ramp4-2 antibody [EPR14338] (ab184571)Anti-Ramp4-2 antibody [EPR14338] (ab184571) at 1/50000 dilution + 293T cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDa
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![Anti-Ramp4-2 antibody [EPR14338] (ab184571)](https://www.abcam.com/ps/products/184/ab184571/Images/ab184571-5-benefits-of-recombinant-antibodies.png)
Anti-Ramp4-2 antibody [EPR14338] (ab184571)