Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Goat monoclonal [RMG01] to Rabbit IgG Fab region
- Suitable for: WB, ELISA
- Reacts with: Rabbit
Overview
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Product name
Anti-Rabbit IgG Fab region antibody [RMG01] -
Description
Goat monoclonal [RMG01] to Rabbit IgG Fab region -
Host species
Goat -
Specificity
ab190495 reacts to the Fab region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG. -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Rabbit
Does not react with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Rabbit IgG.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab190495 purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RMG01 -
Isotype
IgG -
Research areas
Images
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ELISA analysis of different species’ IgG shows ab190495 reacted to the Fab region of Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG as the secondary antibody.
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Titer ELISA analysis of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of ab190495 was used as the primary antibody. An alkaline phosphatase conjugated anti-Goat IgG was used as the secondary antibody.
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All lanes : Anti-Rabbit IgG Fab region antibody [RMG01] (ab190495) at 0.2 µg/ml
Lane 1 : Non-reduced Rabbit IgG
Lane 2 : Reduced Rabbit IgG
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 150 kDa
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