Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464)
Key features and details
- Rabbit polyclonal to PVRL1/NECTIN1 - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PVRL1/NECTIN1 antibody - N-terminal
See all PVRL1/NECTIN1 primary antibodies -
Description
Rabbit polyclonal to PVRL1/NECTIN1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig
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Immunogen
Recombinant fragment within Human PVRL1/NECTIN1 (N terminal). The exact sequence is proprietary.
Database link: Q15223 -
Positive control
- WB: U87-MG whole cell extract; mouse and rat brain tissue extracts. ICC/IF: DIV14 rat E18 primary cortical neurons.
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General notes
This product was previously labelled as PVRL1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464)
DIV14 rat E18 primary cortical neurons stained for PVRL1/NECTIN1 (green) using ab229464 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Cytoskeleton staining: Beta Tubulin 3/Tuj1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Western blot - Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464)All lanes : Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464) at 1/500 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 57 kDa10% SDS-PAGE gel.
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Western blot - Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464)Anti-PVRL1/NECTIN1 antibody - N-terminal (ab229464) at 1/500 dilution + U87-MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Predicted band size: 57 kDa10% SDS-PAGE gel.
