Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)
![Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)](https://www.abcam.com/ps/products/274/ab274325/Images/ab274325-1-anti-pvrigcd112r-antibody-epr23797104-flow-cytometry-293t1.jpg "Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23797-104] to PVRIG/CD112R - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free
See all PVRIG/CD112R primary antibodies -
Description
Rabbit monoclonal [EPR23797-104] to PVRIG/CD112R - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: HEK-293T and human PBMC cells.
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General notes
ab274325 is the carrier-free version of ab256808. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23797-104 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged PVRIG/CD112R construct cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG and ab256808, then fixed with 2% PFA followed by intracellularly staining with anti-myc tag conjugated to AlexaFluor®647.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256808).
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![Flow Cytometry - Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)](https://www.abcam.com/ps/products/274/ab274325/Images/ab274325-2-anti-pvrigcd112r-antibody-epr23797104-flow-cytometry-human-peripheral-blood-mononuclear-cell1.jpg)
Flow Cytometry - Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab256808. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256808).
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![Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)](https://www.abcam.com/ps/products/274/ab274325/Images/ab274325-3-benefits-of-recombinant-antibodies.png)
Anti-PVRIG/CD112R antibody [EPR23797-104] - BSA and Azide free (ab274325)
