Anti-PUMA antibody (ab54288)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PUMA antibody
See all PUMA primary antibodies -
Description
Rabbit polyclonal to PUMA -
Host species
Rabbit -
Specificity
Detects a 23 kD protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16kd band may be seen in some instances, possibly corresponding to PUMAß. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human PUMA (C terminal).
Database link: Q9BXH4 -
Positive control
- Human K562 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab54288)
Immunocytochemistry/Immunofluorescence analysis using ab54288 at 1/125 for 12 hours at 4°C in Heat Shocked HeLa Cells. Sfixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Western blot - Anti-PUMA antibody (ab54288)All lanes : Anti-PUMA antibody (ab54288) at 1/1000 dilution
Lane 1 : Cell lysates prepared form human K562 cells
Lane 2 : Cell lysates prepared from mouse 3T3 cells
Predicted band size: 21 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab54288)Immunocytochemistry/Immunofluorescence analysis using at ab54288 1/125 for 12 hours at 4°C. Tissue: Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-PUMA (CT) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab54288)ab54288 at 1/100 dilution staining PUMA in human K562 cells by Immunocytochemistry.
