Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [PTENS380-NA9] to PTEN (phospho S380)
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-PTEN (phospho S380) antibody [PTENS380-NA9]
See all PTEN primary antibodies -
Description
Rabbit monoclonal [PTENS380-NA9] to PTEN (phospho S380) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human -
Immunogen
Synthetic peptide within Human PTEN (phospho S380). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P60484 -
Positive control
- Flow cyt: A431 and L929 cells; A431 cells (treated with EGF).
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PTENS380-NA9 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
-
ab278557 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278557 (green).
-
Peptide blocking flow cytometric analysis of A431 cells secondary antibody only negative control (light blue) or untreated (red) or treated with EGF (green) or untreated and blocked with phospho-peptide (black) or EGF and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or EGF and blocked with non-phospho peptide (purple) using ab278557 at 0.05 µg/mL.
-
Flow cytometric analysis of A431 cells, untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using ab278557 at 0.1 ug/mL.
-