Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [PTENS380-NA9] to PTEN (phospho S380)
Suitable for: Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-PTEN (phospho S380) antibody [PTENS380-NA9]
See all PTEN primary antibodies
Description

Rabbit monoclonal [PTENS380-NA9] to PTEN (phospho S380)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

See all applications and species data
Immunogen

Synthetic peptide within Human PTEN (phospho S380). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P60484

Run BLAST with Run BLAST with
Positive control
- Flow cyt: A431 and L929 cells; A431 cells (treated with EGF).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

PTENS380-NA9
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)

ab278557 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278557 (green).
Flow Cytometry - Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)

Peptide blocking flow cytometric analysis of A431 cells secondary antibody only negative control (light blue) or untreated (red) or treated with EGF (green) or untreated and blocked with phospho-peptide (black) or EGF and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or EGF and blocked with non-phospho peptide (purple) using ab278557 at 0.05 µg/mL.
Flow Cytometry - Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)

Flow cytometric analysis of A431 cells, untreated and unstained as negative control (blue) or untreated and stained (green) or treated with lambda phosphatase and stained (red) using ab278557 at 0.1 ug/mL.
Anti-PTEN (phospho S380) antibody [PTENS380-NA9] (ab278557)

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