Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [12F2-A10] to PspCas13b - BSA and Azide free
  • Suitable for: WB

Overview

  • Product name

    Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free
    See all PspCas13b primary antibodies

  • Description

    Mouse monoclonal [12F2-A10] to PspCas13b - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    Reacts with Prevotella sp. P5, 125

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273463 is the carrier-free version of ab273440. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273463 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    12F2-A10

  • Isotype

    IgG2a

Images

  • Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

    Exposure time: 10 seconds


    This image was produced using ab273440, the same clone in a different formulation.

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysat

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

    Exposure time: 3 minutes


    This image was produced using ab273440, the same clone in a different formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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