Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)

Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [12F2-A10] to PspCas13b - BSA and Azide free
  • Suitable for: WB

You may also be interested in

Product image
Recombinant Human Adipose Triglyceride Lipase protein (His tag) (ab226233)
Product image
Anti-Survivin antibody [EPR20448] (ab208938)
Product image
Anti-Bcl10 antibody [EPR8587] - BSA and Azide free (ab248953)
Product image
Anti-Carbonic Anhydrase 9/CA9 antibody (ab128883)

Overview

  • Product name

    Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free
    See all PspCas13b primary antibodies
  • Description

    Mouse monoclonal [12F2-A10] to PspCas13b - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Reacts with Prevotella sp. P5, 125

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273463 is the carrier-free version of ab273440. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273463 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    12F2-A10
  • Isotype

    IgG2a

Images

  • Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with PspCas13b expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

    Exposure time: 10 seconds


    This image was produced using ab273440, the same clone in a different formulation.

    Lysates should be made freshly and used in WB immediately to minimize protein degradation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Western blot - Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    All lanes : Anti-PspCas13b antibody [12F2-A10] (ab273440) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysat

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/20000 dilution

    Exposure time: 3 minutes


    This image was produced using ab273440, the same clone in a different formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)
    Anti-PspCas13b antibody [12F2-A10] - BSA and Azide free (ab273463)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Human PTPN1 (PTP1B) knockout HeLa cell lysate (ab257617)

  •  
  • Product image

    Recombinant Rat Profilin 1 protein (ab207134)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.