Anti-PSMD9 antibody [EPR12947] (ab181998)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12947] to PSMD9
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PSMD9 antibody [EPR12947]
See all PSMD9 primary antibodies -
Description
Rabbit monoclonal [EPR12947] to PSMD9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, HeLa, HepG2 and 293 lysates; Human kidney tissue; HepG2 and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12947 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L) -HRP at 1/1000 dilution
Predicted band size: 25 kDa
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).
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Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9 with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.
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Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).
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