Anti-PROX1 antibody [5G10] (ab33219)
![Anti-PROX1 antibody [5G10] (ab33219)](https://www.abcam.com/ps/products/33/ab33219/Images/ab33219-46513-anti-prox1-antibody-5g10-western-blot.jpg "Anti-PROX1 antibody [5G10] (ab33219)")
Key features and details
- Mouse monoclonal [5G10] to PROX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PROX1 antibody [5G10]
See all PROX1 primary antibodies -
Description
Mouse monoclonal [5G10] to PROX1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive result in HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate .
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
5G10 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PROX1 antibody [5G10] (ab33219)All lanes : Anti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml
Lane 1 :Hep G2 nuclear extract lysate (ab14660)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 20 minutes -
![Western blot - Anti-PROX1 antibody [5G10] (ab33219)](https://www.abcam.com/ps/products/33/ab33219/Images/ab33219-46511-anti-prox1-antibody-5g10-western-blot.jpg)
Western blot - Anti-PROX1 antibody [5G10] (ab33219) Image courtesy of an Abreview submitted by Dr. Xia ChenAnti-PROX1 antibody [5G10] (ab33219) at 10 µg/ml + P3 Dentate gyrus whole tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
