Anti-Proteinase Activated Receptor 3/PAR-3 antibody (ab66068)
Key features and details
- Rabbit polyclonal to Proteinase Activated Receptor 3/PAR-3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteinase Activated Receptor 3/PAR-3 antibody
See all Proteinase Activated Receptor 3/PAR-3 primary antibodies -
Description
Rabbit polyclonal to Proteinase Activated Receptor 3/PAR-3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- This antibody gave a positive signal in Human Platelet Whole Cell Lysate.
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General notes
Protein previously labeled as Proteinase Activated Receptor 3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Target
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Function
Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. -
Tissue specificity
Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2151 Human
- Omim: 601919 Human
- SwissProt: O00254 Human
- Unigene: 42502 Human
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Alternative names
- coagulation factor II (thrombin) receptor-like 2 antibody
- Coagulation factor II receptor-like 2 antibody
- F2RL2 antibody
see all
Images
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Western blot - Anti-Proteinase Activated Receptor 3/PAR-3 antibody (ab66068)Anti-Proteinase Activated Receptor 3/PAR-3 antibody (ab66068) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes
Datasheets and documents
References (3)
ab66068 has been referenced in 3 publications.
- Brünnert D et al. Thrombin stimulates gene expression and secretion of IL-11 via protease-activated receptor-1 and regulates extravillous trophoblast cell migration. J Reprod Immunol 132:35-41 (2019). PubMed: 30903857
- Christensen J et al. Protease-activated receptors in the Achilles tendon-a potential explanation for the excessive pain signalling in tendinopathy. Mol Pain 11:13 (2015). PubMed: 25880199
- Wang T et al. Cytokinesis defines a spatial landmark for hepatocyte polarization and apical lumen formation. J Cell Sci 127:2483-92 (2014). Mouse . PubMed: 24706948
Images
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Western blot - Anti-Proteinase Activated Receptor 3/PAR-3 antibody (ab66068)Anti-Proteinase Activated Receptor 3/PAR-3 antibody (ab66068) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes
