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Signal Transduction Signaling Pathway Lipid Signaling Prostaglandins

Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]
    See all Prostaglandin D Synthase (Lipocalin)/PDS primary antibodies
  • Description

    Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab188544)

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal brain lysate; Human prostate tissue; HepG2 cells.
  • General notes

     This product was previously labelled as Prostaglandin D Synthase (Lipocalin)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP12357
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Prostaglandins

Images

  • Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Immunocytochemistry/ Immunofluorescence - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Western blot - Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 21 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

  • Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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