Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19372] to Prolactin/PRL
Suitable for: IHC-Fr, WB, IP
Reacts with: Mouse, Rat

Product name

Anti-Prolactin/PRL antibody [EPR19372]
See all Prolactin/PRL primary antibodies
Description

Rabbit monoclonal [EPR19372] to Prolactin/PRL
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, WB, IPmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat pituitary lysates. IHC-Fr: Mouse pituitary tissue. IP: Mouse pituitary whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19372
Isotype

IgG
Research areas

Western blot - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

All lanes : Anti-Prolactin/PRL antibody [EPR19372] (ab183968) at 1/5000 dilution

Lane 1 : Mouse pituitary lysate
Lane 2 : Rat pituitary lysate
Lane 3 : Mouse uterus lysate
Lane 4 : Rat kidney lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 8 seconds; Lane 2,3,4,5 and 6: 3 second.
Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pituitary tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed cytoplasmic staining on mouse anterior pituitary.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling Prolactin/PRL with ab183968 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed negative staining on mouse kidney.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Prolactin/PRL was immunoprecipitated from 0.35 mg of mouse pituitary whole cell lysate with ab183968 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183968 at 1/5000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse pituitary whole cell lysate, 10µg (Input).

Lane 2: ab183968 IP in Mouse pituitary whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183968 in Mouse pituitary whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-Prolactin/PRL antibody [EPR19372] (ab183968)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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