Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5489] to Progesterone Receptor - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free
    See all Progesterone Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR5489] to Progesterone Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab177930 is the carrier-free version of ab133526. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab177930 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)
    Western blot - Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)
    All lanes : Anti-Progesterone Receptor antibody [EPR5489] (ab133526) at 1/1000 dilution (Purified)

    Lane 1 : MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates
    Lane 2 : Human brain lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 98 kDa
    Observed band size: 118 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133526, the same antibody clone in a different buffer formulation.

    We are unsure how to define the extra bands.

  • Western blot - Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)
    Western blot - Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)
    All lanes : Anti-Progesterone Receptor antibody [EPR5489] (ab133526) at 1/1000 dilution

    Lane 1 : MDA-MB-435 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : T47-D cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 98 kDa
    Observed band size: 118 kDa why is the actual band size different from the predicted?



    This data was developed using ab133526, the same antibody clone in a different buffer formulation.

     

  • Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)
    Anti-Progesterone Receptor antibody [EPR5489] - BSA and Azide free (ab177930)

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