Anti-PRKRIR antibody (ab70334)
Key features and details
- Rabbit polyclonal to PRKRIR
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PRKRIR antibody
See all PRKRIR primary antibodies -
Description
Rabbit polyclonal to PRKRIR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human PRKRIR. Synthetic peptide (Human) corresponding to a region between residues 150 and 200 of human PRKRIR (NP_004696).
Database link: O43422 -
Positive control
- Whole cell lysate from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKRIR antibody (ab70334)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PRKRIR with ab70334 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-PRKRIR antibody (ab70334)All lanes : Anti-PRKRIR antibody (ab70334) at 0.04 µg/ml
Lane 1 : whole cell lysate of HeLa cells at 50 µg
Lane 2 : whole cell lysate of HeLa cells at 15 µg
Lane 3 : whole cell lysate of HeLa cells at 5 µg
Lane 4 : whole cell lysate of NIH3T3 cells at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
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Immunoprecipitation - Anti-PRKRIR antibody (ab70334)IP/WB - 1 mg whole cell lysate of HeLa cells used, and 1/4 of the reaction loaded.
Lane 1: ab70333 used at 3µg/ml lysate for IP.
Lane 2: ab70334 used at 3µg/ml lysate for IP.
Lane 3: IgG control.
ab70334 used at at 1µg/ml for the subsequent WB for all lanes.
Detection: Chemiluminescence with an exposure time of 30 seconds.