Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [6F12] to PREX1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PREX1 antibody [6F12] - BSA and Azide free
    See all PREX1 primary antibodies

  • Description

    Mouse monoclonal [6F12] to PREX1 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: T47D, RAW264.7 and PC-12 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264536 is the carrier-free version of ab264535. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)
    Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)
    All lanes : Anti-PREX1 antibody [6F12] (ab264535) at 1/1000 dilution

    Lane 1 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 186 kDa
    Observed band size: 186 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MDA-MB-231 (PMID: 21172654).

    Exposure time: 59 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264535).

  • Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)
    Western blot - Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)
    All lanes : Anti-PREX1 antibody [6F12] (ab264535) at 1/1000 dilution

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 186 kDa
    Observed band size: 186 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST   

    Exposure time: 180 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264535).

  • Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)
    Anti-PREX1 antibody [6F12] - BSA and Azide free (ab264536)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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