Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
![Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-410316-anti-preb-antibody-epr13252-bsa-and-azide-free-western-blot.jpg "Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13252] to PREB - BSA and Azide free
- Suitable for: IHC-P, ICC, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-PREB antibody [EPR13252] - BSA and Azide free
See all PREB primary antibodies -
Description
Rabbit monoclonal [EPR13252] to PREB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250431 is the carrier-free version of ab181212. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250431 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13252 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)All lanes : Anti-PREB antibody [EPR13252] (ab181212) at 1/5000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab181212, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-4-anti-preb-antibody-epr13252-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)This data was developed using ab181212, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Immunocytochemistry - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-3-anti-preb-antibody-epr13252-bsa-and-azide-free-immunocytochemistry.gif)
Immunocytochemistry - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)This data was developed using ab181212, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-2-anti-preb-antibody-epr13252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)This data was developed using ab181212, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-1-anti-preb-antibody-epr13252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)This data was developed using ab181212, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)](https://www.abcam.com/ps/products/250/ab250431/Images/ab250431-6-benefits-of-recombinant-antibodies.png)
Anti-PREB antibody [EPR13252] - BSA and Azide free (ab250431)
