Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PREB antibody [EPR13252] (ab181212)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13252] to PREB
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human

Product name

Anti-PREB antibody [EPR13252]
See all PREB primary antibodies
Description

Rabbit monoclonal [EPR13252] to PREB
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HT-1376 and Raji cell lysates; Human colon and Human endometrial adenocarcinoma tissues; HT-1376 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13252
Isotype

IgG
Research areas

Western blot - Anti-PREB antibody [EPR13252] (ab181212)

All lanes : Anti-PREB antibody [EPR13252] (ab181212) at 1/5000 dilution

Lane 1 : HT-1376 cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 45 kDa
Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)

Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)

Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-PREB antibody [EPR13252] (ab181212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com