Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PREB antibody [EPR13252] (ab181212)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13252] to PREB
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PREB antibody [EPR13252]
    See all PREB primary antibodies

  • Description

    Rabbit monoclonal [EPR13252] to PREB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-1376 and Raji cell lysates; Human colon and Human endometrial adenocarcinoma tissues; HT-1376 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13252

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PREB antibody [EPR13252] (ab181212)
    Western blot - Anti-PREB antibody [EPR13252] (ab181212)
    All lanes : Anti-PREB antibody [EPR13252] (ab181212) at 1/5000 dilution

    Lane 1 : HT-1376 cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)
    Immunoprecipitation - Anti-PREB antibody [EPR13252] (ab181212)

    Western blot analysis of immunoprecipitation pellet from Raji cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181212 at 1/50 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)
    Immunocytochemistry/ Immunofluorescence - Anti-PREB antibody [EPR13252] (ab181212)

    Immunofluorescent analysis of HT-1376 cells (paraformaldehyde-fixed, 4%) labeling PREB with ab181212 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PREB antibody [EPR13252] (ab181212)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PREB with ab181212 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PREB antibody [EPR13252] (ab181212)
    Anti-PREB antibody [EPR13252] (ab181212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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