Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6D3] to PRDM1/Blimp1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free
    See all PRDM1/Blimp1 primary antibodies

  • Description

    Rat monoclonal [6D3] to PRDM1/Blimp1 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: NIH/3T3 and RAW 264.7 whole cell lysate. IHC-P: Mouse small intestine tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab252242 is the carrier-free version of ab243146. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252242 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)
    Western blot - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)
    All lanes : Anti-PRDM1/Blimp1 antibody [6D3] (ab243146) at 0.89 µg/ml

    Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 96 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243146).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling PRDM1/Blimp1 with ab243146 at 17.8µg/ml followed by ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on the lymph node and surface epithelial cells in the mouse small intestine (PMID: 21878906). Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243146).

  • Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)
    Anti-PRDM1/Blimp1 antibody [6D3] - BSA and Azide free (ab252242)

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