Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)
![Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)](https://www.abcam.com/ps/products/249/ab249037/Images/ab249037-408068-anti-pras40-antibody-epr6263-2-bsa-and-azide-free-western-blot.jpg "Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6263(2)] to PRAS40 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free
See all PRAS40 primary antibodies -
Description
Rabbit monoclonal [EPR6263(2)] to PRAS40 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249037 is the carrier-free version of ab151719 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249037 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6263(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)All lanes : Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab151719, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab151719 observed at 27 kDa. Red - loading control, ab130007, observed at 124 kDa.
ab151719 was shown to recognize PRAS40 in wild-type HAP1 cells as signal was lost at the expected MW in AKT1S1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and AKT1S1 knockout samples were subjected to SDS-PAGE. ab151719 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)](https://www.abcam.com/ps/products/249/ab249037/Images/ab249037-3-anti-pras40-antibody-epr6263-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)This data was developed using ab151719, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRAS40 with ab151719 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Western blot - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)](https://www.abcam.com/ps/products/249/ab249037/Images/ab249037-402311-anti-pras40-antibody-epr6263-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)All lanes : Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SHSY5Y cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?This data was developed using ab151719, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)](https://www.abcam.com/ps/products/249/ab249037/Images/ab249037-1-anti-pras40-antibody-epr6263-2-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037) -
![Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)](https://www.abcam.com/ps/products/249/ab249037/Images/ab249037-5-benefits-of-recombinant-antibodies.png)
Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (ab249037)